| Field | Specification |
|---|---|
| Mfr No | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells. |
| Product Type | |
| Purity | |
| Storage | |
| UniProt # |
Product Overview
Research-grade Peptide Reagent: Human IL2RA (CD25) protein (Recombinant) (STJP000332).
Target & Specificity
| Gene Symbol | IL2RA |
|---|---|
| NCBI Gene ID | 3559 |
| UniProt ID | P01589 |
| Immunogen | Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells. |
| Immunogen Region (aa) | ECD |
| Antibody Production Host | HEK293 |
| Conjugate | Unconjugated |
This peptide is designed for use specifically with the paired STJ-series antibody against the same target epitope. Cross-reactivity with other targets has not been evaluated and should be determined empirically.
Specifications
| Purity | >97%, as determined by SDS-PAGE and HPLC |
|---|---|
| Formulation | 0.2 Mu m filtered PBS solution, pH7.4. |
| Storage | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. |
| Validated Applications | Western Blot|Immunodepletion |
| Recommended Dilution | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
Recommended Use
For WB: load 5–20 ng per lane alongside experimental samples to confirm antibody function and expected band size. For IHC: apply to a positive-control tissue section at the concentration recommended for the paired antibody.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJP000332UniProtP01589
Load 5–20 ng per WB lane alongside experimental samples to confirm anti-IL2RA antibody performance. For IHC, apply to a positive-control tissue section alongside experimental sections. Pair with antibody STJP000332 for matched validation.
For WB: 5–20 ng per lane produces a clear band under standard conditions. For IHC: use the concentration specified on the antibody datasheet. If no signal: confirm antibody is within expiry and antigen retrieval conditions are optimal.
This peptide is designed to pair with antibody STJP000332. Immunogen region: Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells.. Using with a different antibody clone may not produce blocking results if epitopes differ.
Validated for Western Blot, Immunodepletion specificity controls. Performance in other applications (ELISA, IP) requires independent validation.
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles.. Purity: >97% by HPLC. Prepare single-use aliquots to avoid freeze-thaw; stable ≥12 months at −20 °C when lyophilized.
Looking for a custom format, bulk quantity, or specialized formulation? We offer flexible sourcing options. Contact us to discuss your specific requirements.
