| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
The human induced pluripotent stem cell (iPSCs) line is derived from human foreskin cells through reprogramming technology. iPSCs were reprogrammed using OCT4, SOX2, KLF4, and MYC via retroviral transduction. No feeder cells are required for culture.
Key elements and design rationale
- Model identity: Human Induced Pluripotent Stem (iPS) Cells is supplied as an engineered cell line derived from Human skin.
- Growth properties: Adherent, epithelial-like, spherical colonies
- Growth conditions: Matrigel (Corning, Cat. No.: 354277)* 1:100 coated 6-well plates are recommended for optimal cell culture. PriGrow X Series Medium (TM9996), 37.0°C, 5% CO₂. Will require addition of fresh 10 μM ROCK Inhibitor Y-27632 (TM131) in culture. Do not add antibiotics. *Do not re-use Matrigel more than three times; excessive reuse may reduce cell adherence.
- Product format: Frozen, BSL-2
This cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor/background information is available for contextual interpretation.
Biological background
This model supports studies in cell fate, differentiation, and expansion. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor/background information provided for this product: Male, Neonatal.
Research relevance and current trends
- Engineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.
- Reporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.
- Expression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.
Common research applications
- Expansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.
- Comparative experiments that examine changes in morphology, marker expression, or functional readouts over time.
- Assay development in culture systems where media composition and substrate selection influence interpretation.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Matrigel (Corning, Cat. No.: 354277)* 1:100 coated 6-well plates are recommended for optimal cell culture. PriGrow X Series Medium (TM9996), 37.0°C, 5% CO₂. Will require addition of fresh 10 μM ROCK Inhibitor Y-27632 (TM131) in culture. Do not add antibiotics. *Do not re-use Matrigel more than three times; excessive reuse may reduce cell adherence.
- Split Ratio: 1:6 to 1:8
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5-6 ml of pre-warmed, complete growth media. Centrifuge cells at 300xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into coated dishes.
- Add fresh10 μM ROCK Inhibitor Y-27632 to the medium.
- Incubate the cells at the recommended conditions. Change the complete medium the day after thawing and allow time for cell recovery. In 6-well plates, maintain 6-10 cell clusters per field of view, with density not exceeding 80%. If uneven distribution occurs, passage the cells to prevent differentiation.
🔬 Subculture Protocol
Volumes given below are for a 6-well plate; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the colony edges become bright, and density reaches 80-90%.
- Aspirate the culture media, and add 2-3ml of pre-warmed Instant Select & Dissociation Reagent (TM097) to the culture vessel and incubate at 37°C for 1-2 minutes until cell clusters detach.
- Observe the cells under a microscope to confirm detachment (typically within 1-2 minutes).
- Neutralize by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 300xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant. Gently pipette cell suspensions (10-20 times); avoid complete dissociation into single cells with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new coated culture vessels, as desired.
- Add fresh10 μM ROCK Inhibitor Y-27632 to the medium.
- Incubate the cells at the recommended conditions. Recommend to change medium daily.
How should I handle live cells once I receive them?
Please refer to our Cell Handling and Thawing Guidelines for detailed instructions on receiving, thawing, and culturing live cells:
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
What is your warranty or return policy?
Our warranty and return policy is outlined in abm’s Terms and Conditions, including details on product quality, limitations, and claims.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
The number of times cells can divide depends on the cell type:
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Takahashi, K., & Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126(4), 663–676. https://doi.org/10.1016/j.cell.2006.07.024
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., Slukvin, I. I., & Thomson, J. A. (2007). Induced pluripotent stem cell lines derived from human somatic cells. Science (New York, N.Y.), 318(5858), 1917–1920. https://doi.org/10.1126/science.1151526
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
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