| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | EDG2; GPR26; Gpcr26; LPA1; Mrec1.3; VZG1; edg-2; rec.1.3; vzg-1; OTTHUMP00000021904|endothelial differentiation; lysophosphatidic acid G-protein-coupled receptor; 2|ventricular zone gene 1 |
| Product Type | |
| Sensitivity | |
| UniProt # |
Product Overview
Sandwich ELISA KitLysophosphatidic acid receptor 1 is a G protein-coupled receptor which binds the lipid signaling molecule lysophosphatidic acid (LPA). Hence this receptor is also known as LPA1.The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platele…
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate LPAR1 in samples. An antibody specific for LPAR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPAR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPAR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avid…
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
Related Products
Browse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.
This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is 0.312-20 ng/mL. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
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