| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | LPCAT1; AYTL2; FLJ12443; FLJ41609; PFAAP3; lpcat; LPC acyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 1; acyltransferase like 2; phosphonoformate immuno-associated protein 3; regulated by phos |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT1. No significant cross-reactivity or interference between Human LPCAT1 and analogues was observed.
Background
Lysophosphatidylcholine (LPC) acyltransferase (LPCAT) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis.
deduced 534-amino acid mouse and human proteins contain 3 transmembrane domains, several motifs conserved in members of the LPCAT family, a putative EF hand domain, and a C-terminal endoplasmic reticulum (ER) retention signal. They share about 88% amino acid identity. Real-time RT-PCR of mouse tissues showed that Lpcat1 was expressed at highest levels in lung, followed by spleen and brain. Other tissues had low Lpcat1 levels. Confocal microscopy detected epitope-tagged Lpcat1 mainly in the ER and Golgi of transfected cells.
This Lysophosphatidylcholine acyltransferase 1 (LPCAT1) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Lysophosphatidylcholine acyltransferase 1 (LPCAT1) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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