| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | MAST3; KIAA0561 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST3. No significant cross-reactivity or interference between Human MAST3 and analogues was observed.
Background
By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned MAST3, which they designated KIAA0561. The transcript contains repetitive elements in its 3-prime UTR, and the deduced 1,308-amino acid protein shares significant similarity with mouse Mast205 (MAST2) and human MAST4. RT-PCR detected variable MAST3 expression in all tissues examined, with highest levels in kidney, brain, and small intestine.Valiente et al. (2005) showed that the C-terminal tail of human PTEN bound to the PDZ domains of rat Magi2, Magi3, and Dlg (DLG1), mouse Sast (MAST1) and Mast205, and human MAST3. Interaction of PTEN with Magi2 increased PTEN protein stability, and interaction of PTEN with the MAST kinases facilitated phosphorylation of PTEN by these kinases.
This Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Microtubule-associated serine/threonine-protein kinase 3 (MAST3) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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