| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | MAPKBP1; MGC138851; MGC138852; mitogen activated protein kinase binding protein 1; mitogen-activated protein kinase binding protein 1-like |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Mitogen-activated protein kinase-binding protein 1 (MAPKBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPKBP1. No significant cross-reactivity or interference between Human MAPKBP1 and analogues was observed.
Background
MAPKBP1 is expressed at high level, 1.7 times the average gene in this release. MAPKBP1 contains 40 different introns (39 gt-ag, 1 gc-ag). Transcription produces 20 different mRNAs, 14 alternatively spliced variants and 6 unspliced forms. There are 6 probable alternative promotors, 6 non overlapping alternative last exons and 2 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 26 cassette exons, overlapping exons with different boundaries, alternative splicing or retention of 10 introns. 1495 bp of this gene are antisense to spliced gene plargley, raising the possibility of regulated alternate expression. There are 2 articles specifically referring to this gene in PubMed. Proteins are expected to localize in nucleus.
This Mitogen-activated protein kinase-binding protein 1 (MAPKBP1) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Mitogen-activated protein kinase-binding protein 1 (MAPKBP1) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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