| Field | Specification |
|---|---|
| UniProt # | |
| Applications | |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.47 ng/mL |
|---|---|
| Detection Range | 0.78-50 ng/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.47 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant MMP-2 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
These two kits can be used for the detection of your cell supernatant. However, ELISA detection of cell supernatants is affected by factors such as media components, cell growth, and external drug stimulation conditions, and it is recommended that you conduct a preliminary experiment before the formal experiment. Gelatin enzyme spectrometry uses the reversible combination of SDS and MMPs in the sample to separate MMP-2 and MMP-9 in the sample, and then restores the activity of both through the bivalent metal ion buffer system. This test method is specifically aimed at the activity detection of MMP-2 and MMP-9. ELISA detects the content (that is, concentration) of the tested substance through the binding reaction of antigen and antibody, that is, captures the MMP-2 and MMP-9 of the sample through the specific antibodies of MMP-2 and MMP-9. The experimental principles of the two are different, and the activity of MMP-2 and MMP-9 cannot be detected by ELISA.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
What is the range of enzyme activity of your IL-2 freeze-dried powder
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance (2019) JOURNAL OF HEPATOLOGY. 10.1016/j.jhep.2019.05.013
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers (2021) Nature Communications. 10.1038/s41467-021-23789-3
Guizhi-Fuling Wan suppresses carcinoma-associated mesenchymal stem cells-induced ovarian cancer progression via STAT3 inhibition (2025) PHYTOMEDICINE. 10.1016/j.phymed.2025.157475
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis (2023) BIOMEDICINE & PHARMACOTHERAPY. 10.1016/j.biopha.2023.114289
High levels of soluble CD73 unveil resistance to BRAF inhibitors in melanoma cells (2024) BIOMEDICINE & PHARMACOTHERAPY. 10.1016/j.biopha.2024.117033
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases (2021) Cells. 10.3390/cells10123506
Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction (2022) INDUSTRIAL CROPS AND PRODUCTS. 10.1016/j.indcrop.2022.115164
Role of Adipose Tissue Hormones in Pathogenesis of Cryptoglandular Anal Fistula (2024) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms25031501
Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells (2023) Marine Drugs. 10.3390/md21030156
Effects of olive leaf extract supplementation on systemic markers of tissue aging and remodeling in postmenopausal women: a randomized controlled trial with exploratory skin outcomes (2025) Frontiers in Nutrition. 10.3389/fnut.2025.1670194
The Therapeutic Potential of Adipose-Derived Mesenchymal Stem Cell Secretome in Osteoarthritis: A Comprehensive Study (2024) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms252011287
Influence of CReatine Supplementation on mUScle Mass and Strength After Stroke (ICaRUS Stroke Trial): A Randomized Controlled Trial (2024) Nutrients. 10.3390/nu16234148
The Influence of Bariatric Surgery on Matrix Metalloproteinase Plasma Levels in Patients with Type 2 Diabetes Mellitus (2024) Biomolecules. 10.3390/biom14121633
Regulatory Ability of Lactiplantibacillus plantarum on Human Skin Health by Counteracting In Vitro Malassezia furfur Effects (2023) Journal of Fungi. 10.3390/jof9121153
MMP2 Polymorphism Affects Plasma Matrix Metalloproteinase (MMP)-2 Levels, and Correlates with the Decline in Lung Function in Hypersensitivity Pneumonitis Positive to Autoantibodies Patients (2019) Biomolecules. 10.3390/biom9100574
Hypoxia-induced phenotypic transformation of scleral fibroblasts promotes activation of the adhesion patch pathway through paracrine effects leading to choroidal damage (2025) BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. 10.1016/j.bbamcr.2025.119992
Kisspeptin-10 increases collagen content in the myocardium by focal adhesion kinase activity (2023) Scientific Reports. 10.1038/s41598-023-47224-3
Melatonin increases collagen content accumulation and Fibroblast Growth Factor-2 secretion in cultured human cardiac fibroblasts (2023) Pharmacological Reports. 10.1007/s43440-023-00490-4
PHEMA-based Cryogels as 3D Placental Scaffolds (2023) PROCESS BIOCHEMISTRY. 10.1016/j.procbio.2023.08.005
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways (2021) JOURNAL OF ETHNOPHARMACOLOGY. 10.1016/j.jep.2021.114791
Metformin attenuates lens epithelial cell senescence by suppressing cGAS-STING via SIRT1-PGC-1α-mediated mitochondrial fission (2026) EXPERIMENTAL GERONTOLOGY. 10.1016/j.exger.2026.113157
Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain (2022) Spine Journal. 10.1016/j.spinee.2022.01.012
Anthraquinone-bridged diruthenium(II) complexes inhibit migration and invasion of human hepatocarcinoma MHCC97-H cells (2017) Inorganic Chemistry Frontiers. 10.1039/C7QI00149E
Cohort study exploring the association of cerebrospinal fluid metalloprotease levels and Microbiological characteristics to cerebral vasculitis complication in Pneumococcal meningitis (2025) Scientific Reports. 10.1038/s41598-025-99883-z
Isatin, a monoamine oxidase inhibitor, sensitizes resistant breast cancer cells to tamoxifen via MAO-A/HIF1α/MMPs modulation (2025) RSC Medicinal Chemistry. 10.1039/D5MD00694E
