| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
This novel myotonic dystrophy type 2 (DM2) fibroblast cell line is useful for investigations regarding type 2 myotonic dystrophy. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Skin
- Growth properties: Adherent, fibroblast
- Format: Frozen
- Seeding guidance: 200,000 - 1,000,000 cells/cm²
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.
Fibroblast-based disease models are commonly used to study cell-autonomous phenotypes, stress responses, extracellular matrix remodeling, and pathway changes associated with inherited disorders.
Research relevance and current trends
- Patient-derived primary cells are used to connect genotype to measurable cellular phenotypes in vitro.
- Comparative studies often evaluate disease-relevant stress, signaling, and transcriptomic changes across matched controls.
- Cryopreserved donor-derived fibroblasts support repeatable expansion and banking for longitudinal assay development.
Common research applications
- Compare disease-associated phenotypes with control fibroblasts under matched culture conditions.
- Measure transcriptional, metabolic, or signaling changes following perturbation.
- Use as a donor-derived cell source for reprogramming, co-culture, or mechanistic follow-up studies.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 20% FBS + 1% Non-Essental Amino Acids (TM068) + 1% HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Seeding Density (cells/cm²): 200,000 - 1,000,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
🔬 Subculture Protocol
Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.
- Aspirate the culture media, and add 2-3ml of pre-warmed Gentle Dissociation Solution (TM080) to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize the Gentle Dissociation Solution (TM080) by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Jenquin, J. R., O'Brien, A. P., Poukalov, K., Lu, Y., Frias, J. A., Shorrock, H. K., Richardson, J. I., Mazdiyasni, H., Yang, H., Huigens, R. W., 3rd, Boykin, D., Ranum, L. P. W., Cleary, J. D., Wang, E. T., & Berglund, J. A. (2022). Molecular characterization of myotonic dystrophy fibroblast cell lines for use in small molecule screening. iScience, 25(5), 104198. https://doi.org/10.1016/j.isci.2022.104198
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
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