| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Range | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Serum, Plasma, Tissue Homogenates, Other Biological Fluids |
| Sensitivity | |
| Storage | |
| UniProt # |
Assay Principle
This Sandwich ELISA Kit uses a two-site sandwich immunoassay format. A capture antibody specific to PLA2G5 (Phospholipase A2, Group V) is pre-coated on the microplate. Standards and samples are added and any PLA2G5 (Phospholipase A2, Group V) present is captured. A biotinylated detection antibody is added, followed by Streptavidin–HRP conjugate. TMB substrate produces color proportional to PLA2G5 (Phospholipase A2, Group V) concentration; the reaction is stopped and absorbance read at 450 nm. Sample concentrations are interpolated from a standard curve.
Performance Specifications
| Sensitivity | 0.105ng/mL |
|---|---|
| Detection Range | 0.312-20ng/mL |
| Total Assay Time | 4.5 hours |
| Compatible Sample Types | Serum, Plasma, Tissue Homogenates, Other Biological Fluids |
| Species Reactivity | Human |
| Storage | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
✓ Research-Grade Validation
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, Plasma, Tissue Homogenates. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.105ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 4.5 hours, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant PLA2G5 (Phospholipase A2, Group V) standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
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