| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | LNIR; PRR4; nectin-4; Ig superfamily receptor LNIR|nectin 4 |
| Product Type | |
| Sensitivity | |
| UniProt # |
Product Overview
Sandwich ELISA KitPoliovirus receptor-like proteins (PVRLs), such as PVRL4, are adhesion receptors of the immunoglobulin superfamily and function in cell-cell adhesion (Reymond et al., 2001). By database analysis, Reymond et al. (2001) identified the PVRL4 gene, which encodes a 510-amino acid protein with a predicted molecular mass of 55.5 kD. It contains 1 predicted transmembrane domain, followed by a 139-residue cytoplasmic sequence. Human and murine PVRL4 share 92% amino acid identity. Northern blot analysis s…
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PVRL4 in samples. An antibody specific for PVRL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPVRL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PVRL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avid…
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | 125.00-8000 pg/mL |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
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Browse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.
This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is 125.00-8000 pg/mL. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
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