| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Human Primary Bladder Smooth Muscle Cells are isolated from healthy human bladder tissue and exhibit the characteristic spindle-shaped morphology and contractile phenotype of smooth muscle cells. These cells provide a physiologically relevant in vitro model for studying bladder smooth muscle function, contraction, tissue remodeling, and mechanisms underlying urinary tract disorders such as overactive bladder and bladder dysfunction. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Bladder
- Growth properties: Adherent, elongated with tapered ends
- Format: Frozen
- Seeding guidance: 20,000 cells/cm²
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth.
Smooth muscle cells regulate vessel tone, extracellular matrix remodeling, and responses to growth factors, cytokines, and mechanical cues. In culture, they are often used to study contractile-to-synthetic state transitions.
Research relevance and current trends
- Studies frequently examine how inflammatory, mechanical, or metabolic cues alter smooth muscle phenotype and matrix production.
- Primary vascular smooth muscle cells are used to model remodeling pathways relevant to stenosis, calcification, and vascular injury.
- Comparative donor and vessel-source studies help capture regional heterogeneity across the arterial tree.
Common research applications
- Measure contractile marker expression or phenotype switching after defined perturbations.
- Evaluate proliferation, migration, and matrix-remodeling responses in vascular remodeling models.
- Use in co-culture with endothelial cells or fibroblasts to examine vessel wall signaling.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Human Smooth Muscle Cell Growth Medium Kit (TM108) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells can be expanded for up to 2 passages using a 1:2 split ratio.
- Split Ratio: 1:2
- Seeding Density (cells/cm²): 20,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, wash adherent layed with PBS (1X) and add 2-3ml of pre-warmed 0.05% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
