| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Human Primary Normal Small Intestinal Epithelial Cells are human frozen primary cells from intestine used for barrier biology, transport, host-pathogen interactions, and tissue-specific epithelial response studies. These cells display adherent, epithelial growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Intestine
- Growth properties: Adherent, epithelial
- Format: Frozen
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Human Epithelial Cell Growth Medium Kit (TM106) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Epithelial cells form polarized barriers that regulate transport, secretion, and defense at tissue interfaces. Their phenotype is shaped by cell polarity, junctional organization, and tissue-specific differentiation cues.
Research relevance and current trends
- Primary epithelial models are widely used to examine barrier integrity, cytokine responses, and transport processes under physiologic conditions.
- Researchers often combine epithelial cells with stromal or immune components to model tissue microenvironments more faithfully.
- Donor-matched or disease-context epithelial cells help capture inter-individual heterogeneity in response assays.
Common research applications
- Measure barrier integrity, junctional organization, and polarized responses in vitro.
- Evaluate cytokine, pathogen, or drug responses in tissue-relevant epithelial cultures.
- Use in co-culture or air-liquid interface-adjacent workflows when epithelial context matters.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Human Epithelial Cell Growth Medium Kit (TM106) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 8-10ml of pre-warmed, complete growth media. Centrifuge cells at 120xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 6ml of the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Change media the following day to remove non-adherent cells and replenish nutrients.
- Change media every 24-48 hours, and check cells daily under microscope to verify appropriate cell morphology. Change media every day when cells are >70% confluent. Pre-wash cells with 1X DPBS, No Ca, No Mg (CH110) 1-2 times whenever replacing media.
🔬 Subculture Protocol
Volumes given below are for a T25 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.
- Aspirate the culture media, and wash the adherent layer 1-2 times using 5ml sterile pipette with sterile 1X DPBS, No Ca, No Mg (CH110) to dislodge loosely attached cells and remove fraction. Remove and discard the wash solution from flask.
- Incubate cells with add 2ml of pre-warmed 0.25% Trypsin-EDTA for 3-6 minutes. As soon as cells detach (may require few firm gentle taps) add 8-10ml of complete culture media supplemented with 10% FBS to neutralize the trypsin.
- Plate cells in gelatin precoated flasks and incubate the cells at the recommended conditions.
- Change culture media the following day to remove non-adherent cells and replenish nutrients.
- Change media every 24-48 hours, and check cells daily under microscope to verify appropriate cell morphology. Change media every day when cells are >70% confluent. Pre-wash cells with 1X DPBS, No Ca, No Mg (CH110) 1-2 times whenever replacing media.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
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