| Field | Specification |
|---|---|
| Target | |
| Alternative names | Collagen alpha-1 (I) chain; Alpha-1 type I collagen; COL1A1 |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human collagen alpha-1(I) chain (gene COL1A1) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Collagen α1(I) chain (COL1A1) is the major chain of type I collagen, the most abundant fibrillar collagen in bone, skin, tendon and other connective tissues. It is a standard marker of fibroblasts, osteoblasts and fibrosis, and COL1A1 mutations cause osteogenesis imperfecta.
Kit Components
| Anti-Human COL1A1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human COL1A1 Standard | 2 vials, 10 ng/tube |
| Human COL1A1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human COL1A1 (Gln23–Lys277), expressed in CHO |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 2.9–7.4% (3 samples, n = 16 each) |
| Inter-assay CV | 4.6–7.2% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:2000. In the supplier’s tests, serum and plasma samples measured 594–1000 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.021; 62.5 = 0.109; 125 = 0.199; 250 = 0.314; 500 = 0.623; 1000 = 1.056; 2000 = 1.829; 4000 = 2.496. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.021 | 0.109 | 0.199 | 0.314 | 0.623 | 1.056 | 1.829 | 2.496 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 105 | 360 | 1669 | 78 | 251 | 1684 |
| Standard deviation | 3.03 | 19.01 | 123.34 | 5.65 | 11.55 | 105.59 |
| CV(%) | 2.9% | 5.4% | 7.4% | 7.2% | 4.6% | 6.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Activating reagent preparation
Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml human COL1A1 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well). Add 0.1 ml of each properly diluted sample of human cell culture supernatants, serum or plasma (heparin, EDTA, citrate) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each human COL1A1 standard solution and each sample be measured in duplicate.
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Hua-Mo Yin et al. (2019) Surface Epitaxial Crystallization-Directed Nanotopography for Accelerating Preosteoblast Proliferation and Osteogenic Differentiation. ACS Applied Materials & Interfaces. 10.1021/acsami.9b14800
Zhongjie Tang et al. (2026) Balancing immune evasion and hepatocyte delivery: a hybrid liposomal platform for enhanced nanotherapy in non-alcoholic fatty liver disease. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2026.126657
Xiyan Xiang et al. (2025) Serum Proteomic Profile Based on the TGF-β Pathway Stratifies Risk of Hepatocellular Carcinoma. Liver International. 10.1111/liv.70325
Anissa Lasfar et al. (2025) Effects of olive leaf extract supplementation on systemic markers of tissue aging and remodeling in postmenopausal women: a randomized controlled trial with exploratory skin outcomes. Frontiers in Nutrition. 10.3389/fnut.2025.1670194
Baisong Zhao et al. (2020) Combination of nanolamellae and PDA coating on promoting the long-term adhesion, proliferation, and differentiation of osteoblasts. Polymer. 10.1016/j.polymer.2020.122462
MingJie Wang et al. (2017) The excitotoxity of NMDA receptor NR2D subtype mediates human fetal lung fibroblasts proliferation and collagen production. Toxicology in Vitro. 10.1016/j.tiv.2017.10.008
Yuanbin Li et al. (2024) Enhancement of silymarin solubility and bioactivities using betaine/ascorbyl glucoside DES. New Journal of Chemistry. 10.1039/D4NJ03748K
Rui Jiang et al. (2019) Protective Effects of Ginseng Proteins on Photoaging of Mouse Fibroblasts Induced by UVA. Photochemistry and Photobiology. 10.1111/php.13156
Heng Pan et al. (2025) Effect of chair-side treatment with 172 nm vacuum ultraviolet light on the surface properties of three different implant materials. Materials Research Express. 10.1088/2053-1591/adc787
Zhongsheng Liu et al. (2019) Uncarboxylated osteocalcin promotes osteogenic differentiation of mouse bone marrow–derived mesenchymal stem cells by activating the Erk-Smad/β-catenin signalling pathways. Cell Biochemistry and Function. 10.1002/cbf.3457
