| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SCARA5; FLJ23907; MGC45780; NET33; Tesr; scavenger receptor class A; member 5; testis expressed scavenger receptor |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCARA5. No significant cross-reactivity or interference between Human SCARA5 and analogues was observed.
Background
Scara5, encoding a deduced 491-amino acid protein that shares 88% sequence identity with the 495-amino acid human ortholog. Although overall identity with Scara1 is about 25%, Scara5 and Scara1 share many features, including an N-terminal cytoplasmic domain followed by a predicted transmembrane domain, a spacer region with several putative glycosylation sites, a collagenous domain, a hinge region, and a C-terminal scavenger receptor cysteine-rich domain (SRCR). The SCARA5 protein was predicted to be trimeric, as are other collagenous transmembrane proteins, and this prediction was supported by Western blotting. Based on similarity to other members of this family, the trimer is present on the surface of the plasma membrane and anchored by the transmembrane domain.
This Scavenger receptor class A member 5 (SCARA5) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Scavenger receptor class A member 5 (SCARA5) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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