| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SAA4; C-SAA; CSAA |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Serum amyloid A-4 protein (SAA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SAA4. No significant cross-reactivity or interference between Human SAA4 and analogues was observed.
Background
Studying the protein and the cDNA, Whitehead et al. (1992) identified a new member of the serum amyloid A protein superfamily, designated SAA4, as constitutive apolipoprotein of high density lipoprotein. Watson et al. (1992) analyzed the structure of the gene and the derived protein structure. Steel et al. (1993) demonstrated that the SAA4 gene is located 9 kb downstream of the SAA2 gene (104751) on chromosome 11. Furthermore, it shares the same 5-prime to 3-prime orientation and has the characteristic 4-exon structure of the other members of the SAA superfamily. By fluorescence in situ hybridization using a cosmid probe carrying the SAA2 and SAA4 genes, Sellar et al. (1994) localized these genes to 11p15.1. De Beer et al. (1996) found that in the mouse the Saa4 gene is linked to the serum amyloid A gene family on chromosome 7.
This Serum amyloid A-4 protein (SAA4) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Serum amyloid A-4 protein (SAA4) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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