| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SIGLEC6; CD327; CD33L; CD33L1; CDw327; OBBP1; SIGLEC-6; CD33 antigen-like 1; CDw327 antigen; Obesity-binding protein 1 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC6. No significant cross-reactivity or interference between Human SIGLEC6 and analogues was observed.
Background
The deduced 442-amino acid CD33L protein contains a signal peptide, an N-terminal Ig-like V-domain, and 2 adjacent Ig C2-like domains, followed by a transmembrane region and a cytoplasmic tail. Based on its predicted structure, the authors stated that CD33L belongs to the Ig superfamily and is likely a novel member of the sialoadhesin subfamily. Compared to the original cDNA, this cDNA contains a 176-bp deletion in the coding sequence, resulting in a predicted 342-amino acid protein lacking the transmembrane and cytoplasmic regions. By RT-PCR of placenta RNA, the authors detected both transcripts, although the transcript encoding the membrane-bound isoform, CD33L1, was considerably more abundant.CD33L expression only in the placenta; transcripts of 4 distinct sizes were found, including 1 that was differentially polyadenylated.
This Sialic acid-binding Ig-like lectin 6 (SIGLEC6) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Sialic acid-binding Ig-like lectin 6 (SIGLEC6) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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