| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SLC6A9; DKFZp547A1118; GLYT1; sodium- and chloride-dependent glycine transporter 1; solute carrier family 6 member 9 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A9. No significant cross-reactivity or interference between Human SLC6A9 and analogues was observed.
Background
Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS
This Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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