| Field | Specification |
|---|---|
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| Accession Number | |
| Alternative Names | SLC6A19; B0AT1; FLJ20680; FLJ34635; HND; sodium-dependent amino acid transporter system B0; solute carrier family 6 (neurotransmitter transporter); member 19; solute carrier family 6; system B0 neutr |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A19. No significant cross-reactivity or interference between Human SLC6A19 and analogues was observed.
Background
SLC6A19 is a system B(0) transporter that mediates epithelial resorption of neutral amino acids across the apical membrane in the kidney and intestine.The deduced 634-amino acid protein contains 12 transmembrane domains. In situ hybridization detected Slc6a19 expression in renal cortical proximal tubules and in small intestine villus enterocytes, with highest expression in apical cells. EST database analysis suggested expression in several other tissues and cells. contains 634 amino acids and has 12 predicted transmembrane regions. In a panel of human cDNAs, they detected robust expression of SLC6A19 in kidney and small intestine, with minimal expression in pancreas. SLC6A19 was also expressed in stomach, liver, duodenum, and ileocecum.
This Sodium-dependent neutral amino acid transporter B (SLC6A19) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Sodium-dependent neutral amino acid transporter B (SLC6A19) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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