| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SLC5A5; NIS; TDH1; Na(+)/I(-) cotransporter |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Sodium/iodide cotransporter (SLC5A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC5A5. No significant cross-reactivity or interference between Human SLC5A5 and analogues was observed.
Background
The sodium-iodide symporter (NIS) is an ion pump that actively transports iodide (I-) across the basolateral membrane into thyroid epithelial cells. The NIS a member of the sodium/solute symporter family.Active transport of iodine into the thyroid gland is an important step in the process of iodide organification and the formation of triiodothyronine (T3) and thyroxine (T4). The symporter is stimulated by TSH, thereby increasing iodine uptake and iodination of tyrosine.The pump symports two sodium ions for every iodide ion. This symporter is driven by low internal sodium concentration in the thyroid epithelial cells, via facilitated diffusion, caused by the sodium/potassium ATPase pump.This pump is the target of certain anion based inhibitors such as perchlorate, pertechnetate, and thiocyanate.
This Sodium/iodide cotransporter (SLC5A5) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Sodium/iodide cotransporter (SLC5A5) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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