| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Serum, Plasma, Other Biological Fluids |
| Sensitivity | |
| Storage |
Assay Principle
suPAR (STJE0006654) is a biomarker protein expressed in human tissues and biological fluids. Dysregulation of STJE0006654 has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.
Quantitative measurement of STJE0006654 enables researchers to characterize its abundance in Serum, Plasma and correlate levels with experimental conditions. This kit recognizes Human suPAR in samples. No significant cross-reactivity or interference between Human suPAR and analogues was observed.
This sandwich ELISA kit provides a validated, reproducible format for STJE0006654 quantification across a defined standard curve range of 0.156~10ng/mL. The assay supports experimental workflows in Cell Biology research.
Kit Components
Each kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):
Pre-coated 96-well microplate (capture antibody), HRP-conjugated detection antibody, recombinant standard protein, sample/standard diluent, concentrated wash buffer, TMB substrate solution, stop solution, and product manual.
Validated Lot Colorimetric ReadoutPerformance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.156~10ng/mL |
| Sensitivity (LLOD) | 0.094ng/mL |
| Validated Sample Types | Serum, Plasma |
| Total Assay Time | 3.5 hours |
| Detection Method | Colorimetric (450 nm / 540–570 nm reference) |
| Plate Format | 96-well microplate |
| Reactivity | Human |
| Storage | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual |
Assay Procedure Summary
Add samples and standards to pre-coated capture antibody wells and incubate. Wash, add HRP-conjugated detection antibody, and incubate. Wash again, add TMB substrate, and stop the reaction. Read absorbance at 450 nm (reference 540–570 nm). Interpolate sample concentrations from the standard curve using a 4-parameter logistic (4-PL) fit. Total protocol time: approximately 3.5 hours.
Pre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJE0006654SpeciesHuman
This kit is validated for serum and plasma. Centrifuge samples at ≥1,000 × g for 15 min to remove particulates. Avoid hemolyzed, lipemic, or heat-inactivated samples. Pre-dilute samples within the linear range before committing full plates.
Minimum detectable concentration: 0.094ng/mL. Quantitative standard curve range: 0.156~10ng/mL. Samples above the upper limit must be diluted; near-lower-limit samples require duplicate measurement.
Total protocol time is approximately 3.5 hours of hands-on and incubation time. Pre-warm all reagents and prepare dilution series in advance. Set up a timer for each incubation step to maintain consistent timing across plates.
A standard microplate spectrophotometer set to 450 nm (reference: 540–570 nm) is required. An automated plate washer reduces wash-step variability. Calibrate and warm up your reader before measuring. Blank using the zero-standard or buffer-only well before constructing the standard curve.
Recommended storage: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.. Reconstituted standard is stable at 4 °C for ≤1 week; prepare fresh for critical experiments.
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