| Field | Specification |
|---|---|
| Target | |
| Alternative names | Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1;TIMP-1;TIMP1;CLGI, TIMP; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: C24-A207 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human metalloproteinase inhibitor 1 (gene TIMP1) in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
TIMP-1 is a secreted tissue inhibitor of metalloproteinases that binds the catalytic zinc site of many matrix metalloproteinases, including MMP-9, and blocks their activity. It also has growth factor–like, MMP-independent effects on cell survival and proliferation. TIMP-1 is studied in extracellular matrix remodeling, fibrosis, inflammation and cancer.
Kit Components
| Anti-Human TIMP1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human TIMP1 Standard | 2 vials, 10 ng/tube |
| Human TIMP1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant human TIMP1 (Cys24–Ala207), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.8–7.0% (3 samples, n = 16 each) |
| Inter-assay CV | 7.0–8.4% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1000. In the supplier’s tests, serum samples measured 71.8–137 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.036; 15.6 = 0.095; 31.2 = 0.168; 62.5 = 0.295; 125 = 0.581; 250 = 0.952; 500 = 1.45; 1000 = 1.923. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.036 | 0.095 | 0.168 | 0.295 | 0.581 | 0.952 | 1.45 | 1.923 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 194 | 429 | 28 | 209 | 433 |
| Standard deviation | 1.59 | 12.22 | 29.17 | 2.12 | 14.63 | 36.37 |
| CV (%) | 6.8% | 7% | 6.8% | 7.6% | 7% | 8.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Li Ailing et al. (2018) LincRNA 1700020I14Rik alleviates cell proliferation and fibrosis in diabetic nephropathy via miR-34a-5p/Sirt1/HIF-1α signaling. Cell Death & Disease. 10.1038/s41419-018-0527-8
Daria Sollazzo et al. (2016) Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study. Oncotarget. 10.18632/oncotarget.9949
Mengqi Zhang et al. (2024) Dachengqi decoction dispensing granule ameliorates LPS-induced acute lung injury by inhibiting PANoptosis in vivo and in vitro. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118699
Liu Yun et al. (2024) SERPINA3, FGA, AGP1, ITIH3 and SAA1 as novel biomarkers for eosinophilic granulomatosis with polyangiitis diagnosis and activity assessment. Rheumatology. 10.1093/rheumatology/keae187
Li Rui et al. (2020) PDGFRβ-targeted TRAIL specifically induces apoptosis of activated hepatic stellate cells and ameliorates liver fibrosis. Apoptosis. 10.1007/s10495-019-01583-3
Vincenzo Di Stefano et al. (2022) Metalloproteinases and Tissue Inhibitors in Generalized Myasthenia Gravis. A Preliminary Study. Brain Sciences. 10.3390/brainsci12111439
Cosentino Giuseppe et al. (2021) Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy. Neurological Sciences. 10.1007/s10072-021-05314-y
Weiliang Tang et al. (2013) Activation of extracellular signal-regulated kinase 1/2 and Sp1 may contribute to the expression of tissue inhibitor of metalloproteinases-1 induced by transforming growth factor-β1 in human pulmonary arterial smooth muscle cells. Cytotherapy. 10.1016/j.jcyt.2013.08.009
de Lima Ana Carolina Bastos et al. (2023) Predictors of Trypanosoma cruzi PCR positivity in patients with chronic Chagas disease. Memorias Do Instituto Oswaldo Cruz. 10.1590/0074-02760230115
Jifeng Liu et al. (2014) Effect of Low-Dose, Long-Term Roxithromycin on Airway Inflammation and Remodeling of Stable Noncystic Fibrosis Bronchiectasis. Mediators of Inflammation. 10.1155/2014/708608
