| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | TIPRL; RP1-69E11.1; MGC3794; TIP; TIP41; dJ69E11.3; CG9578-like; TOR signalling pathway regulator-like; putative MAPK activating protein; yeast YPR037W and worm C02C2.6 predicted proteins-like |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human TIP41-like protein (TIPRL) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIPRL. No significant cross-reactivity or interference between Human TIPRL and analogues was observed.
Background
TIPRL is an inhibitory regulator of protein phosphatase-2A (PP2A), PP4 , and PP6. TIP isoform-2 did not interact with PP2A, suggesting that the C terminus of TIP is important for PP2A binding. Incubation of PP2A with TIP, but not TIP isoform-2, resulted in dose-dependent inhibition of PP2A phosphatase activity. Cells in which TIP was depleted by small interfering RNA exhibited decreased phosphorylation of a 32-kD substrate of ATM/ATR kinases.
TIP is an inhibitory regulator of PP2A and that the TIP/PP2A complex has a role within the ATM/ATR signaling pathway controlling DNA replication and repair.The predicted protein contains 272 amino acids. Immunoblot analysis showed ubiquitous expression of a 33-kD TIP protein.
This TIP41-like protein (TIPRL) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte TIP41-like protein (TIPRL) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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