| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | TRAM1; PNAS8; PRO1292; TRAM; TRAMP; translocating chain-associating membrane protein; translocation-associating membrane protein 1 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRAM1. No significant cross-reactivity or interference between Human TRAM1 and analogues was observed.
Background
TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
This Translocating chain-associated membrane protein 1 (TRAM1) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Translocating chain-associated membrane protein 1 (TRAM1) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Human. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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