| Field | Specification |
|---|---|
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant Human WIF-1 (Q166K) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly29-Trp379 (Gln166Lys) ) of human WIF-1 (Accession #NP_009122.2) fused with a 6×His tag at the C-terminus. |
| Product Type | |
| Purity | |
| Storage | |
| UniProt # |
Product Overview
Research-grade Peptide Reagent: Human WIF-1 (Q166K) protein (Recombinant) (C-His) (STJP000963).
Target & Specificity
| NCBI Gene ID | 11197 |
|---|---|
| UniProt ID | Q9Y5W5 |
| Immunogen | Recombinant Human WIF-1 (Q166K) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly29-Trp379 (Gln166Lys) ) of human WIF-1 (Accession #NP_009122.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region (aa) | Gly29-Trp379Gln166Lys |
| Antibody Production Host | HEK293 cells |
| Conjugate | Unconjugated |
This peptide is designed for use specifically with the paired STJ-series antibody against the same target epitope. Cross-reactivity with other targets has not been evaluated and should be determined empirically.
Specifications
| Purity | > 97% by SDS-PAGE. |
|---|---|
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM HAc-NaAc, 150mM NaCl, 0.5% CHAPS, pH 6.0. |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Validated Applications | Western Blot|Immunodepletion |
Recommended Use
For WB: load 5–20 ng per lane alongside experimental samples to confirm antibody function and expected band size. For IHC: apply to a positive-control tissue section at the concentration recommended for the paired antibody.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJP000963UniProtQ9Y5W5
Load 5–20 ng per WB lane alongside experimental samples to confirm anti-Human WIF-1 (Q166K) antibody performance. For IHC, apply to a positive-control tissue section alongside experimental sections. Pair with antibody STJP000963 for matched validation.
For WB: 5–20 ng per lane produces a clear band under standard conditions. For IHC: use the concentration specified on the antibody datasheet. If no signal: confirm antibody is within expiry and antigen retrieval conditions are optimal.
This peptide is designed to pair with antibody STJP000963. Immunogen region: Recombinant Human WIF-1 (Q166K) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly29-Trp379 (Gl. Using with a different antibody clone may not produce blocking results if epitopes differ.
Validated for Western Blot, Immunodepletion specificity controls. Performance in other applications (ELISA, IP) requires independent validation.
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.. Purity: >97% by HPLC. Prepare single-use aliquots to avoid freeze-thaw; stable ≥12 months at −20 °C when lyophilized.
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