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| Formulation | |
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| UniProt # |
Scientific Background
Human herpesvirus entry mediator A, also known as HVEM, TNFRSF14, CD270, and HVEA, GenBank Accession No. NM_003820, a.a. 37-202 fused to human IgG1 Fc, with C-terminal Avi-tagTM, expressed in a HEK293 cell expression system. MW = 46 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Product Description
Mammalian (HEK293) ExpressionRecombinant HVEM Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | Q92956 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 37-202 |
| Molecular Weight | 46 kDa |
| Endotoxin | <1 EU/µg |
| Formulation | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 37-202. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Derre, L., et al. J. Clin. Invest. 2010,120(1): 157-167.
- Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.
