Product Overview
Research Use OnlyHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency.
Validated Applications
| Western Blot (WB) | 1:1000~10000 |
|---|---|
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Mouse |
|---|---|
| Species Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | Predicted band size:102kDa Observed band size:102kDa |
| Catalog No. | MB0140 |
| UniProt | P19367 |
| Alternative Names | Hexokinase-1; Hexokinase 1; Brain form hexokinase; Hexokinase type I; HK I; HK1 |
Immunogen / Antigen
Recombinant full length Human HXK I.
Reactivity Notes
This antibody detects endogenous levels of Hexokinase-1 and does not cross-react with related proteins
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:1000~10000 ICC: 1:100~500
This antibody has been confirmed reactive against: Human. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant full length Human HXK I.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
