| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Biological Activity | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Yeast cells. |
| Species | |
| Storage | |
| Target |
Product Description
Isocitrate Dehydrogenase-1 Yeast Recombinant
Overview
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
Biological Activity
The specific activity was found to be 115 U/mg.
Specifications
| Purity | Greater than 90% as determined by SDS-PAGE. |
|---|---|
| Formulation | One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | Yeast cells. |