| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IDH3B recombinant protein (Position: A32-D362) was used as the immunogen for the IDH3B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IDH3B Antibody / Isocitric dehydrogenase subunit beta is a anti-IDH3B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: IDH3B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IHC, WB
Biological background
Functionally, IDH3B antibody identifies a 389-amino-acid mitochondrial matrix protein that forms part of the heterotetrameric IDH3 enzyme complex, along with alpha and gamma subunits (IDH3A and IDH3G). IDH3B provides structural stability and catalytic support, maintaining proper enzyme kinetics and cofactor affinity. This enzyme's reaction is irreversible under physiological conditions and represents a key regulatory step in the TCA cycle, linking carbohydrate, lipid, and amino acid metabolism.
The IDH3B gene is located on chromosome 20p13 and encodes a protein expressed in energy-demanding tissues such as heart, skeletal muscle, and brain. IDH3B ensures efficient NADH production for ATP generation and contributes to metabolic integration between glycolysis and mitochondrial respiration. Its expression adapts to cellular energy status and oxygen availability, aligning mitochondrial metabolism with energy demand.
Mutations or reduced expression of IDH3B can impair mitochondrial respiration, resulting in metabolic dysfunction and neurodegenerative phenotypes. In contrast to NADP+-dependent IDH1 and IDH2, IDH3B is strictly NAD+-dependent and operates exclusively within mitochondria. Dysregulation of IDH3B expression has been observed in cancers and inherited mitochondrial disorders, where altered TCA cycle flux influences redox homeostasis and biosynthetic metabolism.
IDH3B antibody is widely used in metabolism, mitochondrial biology, and enzymology research. It is suitable for western blotting, immunofluorescence, and metabolic profiling assays to detect IDH3B in tissue or cell lysates. This antibody aids in studying mitochondrial energy metabolism, redox regulation, and enzyme complex formation. In disease models, IDH3B serves as a biomarker for mitochondrial dysfunction and altered oxidative metabolism.
Structurally, IDH3B forms part of the IDH3 heterotetramer, contributing to the active site environment through hydrogen-bonding and metal ion coordination. Post-translational modifications, including phosphorylation and acetylation, regulate its catalytic efficiency and protein-protein interactions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
