| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IDNK recombinant protein (Position: Q56-E182) was used as the immunogen for the IDNK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IDNK Antibody / Gluconokinase is a anti-IDNK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: IDNK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, IDNK antibody identifies a 314-amino-acid enzyme that catalyzes the ATP-dependent phosphorylation of gluconate. The reaction product, 6-phosphogluconate, enters the oxidative branch of the pentose phosphate pathway, contributing to NADPH production and ribose biosynthesis. These processes are vital for maintaining redox balance and supporting anabolic metabolism in proliferating cells.
The IDNK gene is located on chromosome 1p36.11 and encodes a kinase belonging to the gluconokinase family. IDNK functions primarily in tissues with active glucose metabolism, such as liver and kidney, where it provides an alternative route for pentose phosphate pathway intermediates. The enzyme's activity is regulated by substrate availability and energy status, integrating carbohydrate metabolism with cellular redox needs.
In metabolic regulation, IDNK contributes to the detoxification of gluconate derived from glucose oxidation and polyol pathway intermediates. It also plays a role in microbial defense and oxidative stress adaptation by maintaining adequate NADPH pools. Altered IDNK expression has been observed in metabolic disorders, cancer, and diabetic tissues, reflecting its role in redox homeostasis and biosynthetic metabolism.
IDNK antibody is widely used in enzymology, metabolism, and redox biology research. It is suitable for immunoblotting, enzyme activity assays, and metabolic flux studies to analyze IDNK expression and function. This antibody supports investigations into pentose phosphate pathway regulation, antioxidant defense, and carbohydrate catabolism. In cancer studies, IDNK detection helps elucidate metabolic rewiring associated with elevated NADPH demand.
Structurally, IDNK adopts an alpha/beta-fold typical of sugar kinases, with conserved ATP-binding and substrate recognition motifs. Its catalytic mechanism involves a sequential binding of ATP and gluconate, followed by phosphoryl transfer.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
