| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human IFT88 was used as the immunogen for the IFT88 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IFT88 Antibody / Intraflagellar transport protein 88 is a anti-IFT88 Rabbit antibody Recombinant Rabbit Monoclonal clone 29I92 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: IFT88
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29I92, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Mutations in IFT88 cause defects in cilia formation and are associated with a spectrum of ciliopathies, including polycystic kidney disease, retinal degeneration, and skeletal abnormalities. Detection with IFT88 antibody is commonly used in model systems to evaluate cilia integrity and to dissect the molecular mechanisms of ciliary dysfunction. In mice, loss of Ift88 leads to abnormal embryonic development, kidney cysts, and lethality, underscoring the essential nature of this protein for vertebrate survival. In humans, IFT88 mutations have been linked to conditions such as short rib thoracic dysplasia and other syndromes characterized by impaired skeletal growth and organ development.
IFT88 plays a role in Hedgehog signaling, a developmental pathway that depends on intact primary cilia. Studies using IFT88 antibody have revealed its contribution to regulating signal transduction, cell differentiation, and tissue patterning. Aberrant Hedgehog activity linked to ciliary defects highlights the importance of IFT88 in development and disease. This protein is also connected to Wnt signaling and planar cell polarity pathways, expanding its relevance in understanding developmental disorders and congenital anomalies.
IFT88 antibody is useful in western blotting, immunofluorescence, and immunohistochemistry. Immunofluorescence staining of IFT88 localizes the protein to basal bodies and ciliary axonemes, providing spatial resolution of its function. Western blotting identifies protein levels in experimental systems ranging from cultured cells to tissues with high ciliary content. Immunohistochemistry further allows visualization of cilia associated expression in organ samples, including kidney, brain, and respiratory epithelium, making it a valuable tool for diverse biological studies.
Research on ciliopathies, kidney disease, developmental signaling, and structural cell biology relies on validated reagents such as IFT88 antibody.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
