| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 13-40 from the human protein was used as the immunogen for the IL-31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL-31 Antibody / Interleukin 31 is an antibody targeting IL-31, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: IL-31 (reported localization: Secreted).
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human.
- Listed applications: WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).
Biological background
Interleukin 31 (IL-31) is a cytokine that plays a critical role in inflammatory responses and allergic reactions in the body. IL-31 is produced by T-helper 2 (Th2) cells, which are involved in the body's response to allergens and parasites. When released, IL-31 binds to receptors on various cells in the body, triggering a cascade of inflammatory responses that can lead to symptoms such as itching, redness, and swelling. Studies have shown that IL-31 is particularly important in the development of atopic dermatitis, a common skin condition characterized by itchy, inflamed skin. In individuals with atopic dermatitis, levels of IL-31 are often elevated in the skin, leading to increased inflammation and itching. Additionally, IL-31 has also been implicated in other inflammatory conditions such as asthma and inflammatory bowel disease, highlighting its importance in the body's immune response.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
