| Field | Specification |
|---|---|
| Biological Activity | |
| Product Format | Cryovial, 1.0 ml (~2 × 10^5 cells) |
| Product Type | |
| Selection Marker | Neomycin (G418) |
| Species | |
| Storage |
Scientific Background
Adipose tissue exists in two functionally distinct forms. Brown adipose tissue (BAT) is the body's primary site of non-shivering thermogenesis, packed with UCP-1–rich mitochondria, while white adipose tissue (WAT) is the principal energy-storage depot and an active endocrine organ secreting adipokines. Immortalized preadipocyte lines from both depots — at young and aged donor stages — provide a matched in vitro panel for studies of adipogenesis, thermogenesis, lipid metabolism, mitochondrial biology, and the metabolic decline associated with aging.
Product Description
This product is a panel of four immortalized mouse preadipocyte cell lines, selectable by Cell Type (brown or white) and Donor Age (young or aged). All lines were derived from C57BL/6 mouse stromal vascular fraction cells, immortalized by retroviral delivery of the SV40 large T-antigen, and enriched by neomycin (G418) selection. They proliferate stably and differentiate into mature adipocytes — UCP-1–positive brown adipocytes or PPARγ+/FABP4+ white adipocytes — making the set ideal for comparative adipocyte aging and metabolism research. Select your line using the options above.
Variant Comparison
| Line (SKU) | Tissue / Depot | Donor Age | Strain | Differentiation marker |
|---|---|---|---|---|
| Brown, Young (BHC10000001) | Interscapular brown adipose tissue (iBAT) | 1 day (neonatal) | C57BL/6J | UCP-1–positive brown adipocytes |
| Brown, Old (BHC10000002) | Interscapular brown adipose tissue (iBAT) | 27 months (aged) | C57BL/6J | UCP-1–positive brown adipocytes |
| White, Young (BHC10000003) | Subcutaneous white adipose tissue (sWAT) | Young | C57BL/6 | PPARγ+/FABP4+ white adipocytes |
| White, Old (BHC10000004) | Subcutaneous white adipose tissue (sWAT) | 18 months (aged) | C57BL/6J | PPARγ+/FABP4+ white adipocytes |
Shared Specifications
| Species | Mouse (Mus musculus) |
|---|---|
| Strain | C57BL/6 / C57BL/6J |
| Sex | Male |
| Cell Type | Preadipocyte (brown or white, by variant) |
| Immortalization | SV40 large T-antigen (retroviral); neomycin/G418 selectable marker |
| Growth Properties | Adherent |
| Biosafety Level | BSL-2 |
| Format | Cryovial, 1.0 ml (~2 × 105 cells) |
| Storage | Liquid nitrogen (≤ –160 °C) |
Quality Control & Validation
Sterility: Pass Mycoplasma: Pass Post-freeze viability ~80%
Passage number varies by line (3–5). Recovery: seed into a T25 flask at 2 × 105 cells; reaches ~90% confluence within ~4 days. A lot-specific Certificate of Analysis is provided with each vial (Certificates for the brown young, brown aged, and white aged lines are attached under Documents / References).
Usage Notes
Complete growth medium: DMEM/F-12 supplemented with 10% fetal bovine serum (FBS) and 100 µg/ml Primocin. Filter-sterilize through a 0.2 µm filter; store up to 6 months at 4 °C.
Growth & culture conditions: Maintain at 37 °C in a humidified 5% CO2 incubator. Refresh growth medium every other day. Subculture at 80–90% confluence using a 1:3–1:4 split ratio.
Subculture / propagation: Aspirate medium and wash once with 1× PBS. Add 0.25% trypsin-EDTA to cover the cell surface and incubate at 37 °C for 2–3 min until cells round up. Neutralize with growth medium, collect, and centrifuge for 3 min at 400 × g. Resuspend and reseed at a 1:3–1:4 ratio.
Thawing: Thaw the cryovial in a 37 °C water bath for 2–3 min. Centrifuge 3 min at 400 × g (4 °C) and remove the freezing medium. Resuspend in growth medium and seed to reach ~40–50% confluence. Culture at 37 °C, 5% CO2.
Cryopreservation medium: 90% FBS and 10% DMSO (filter-sterilized). Store in liquid nitrogen for long-term preservation.
Wu, X., Elsaid, S., Levet, F., Li, W., & Tee, S. S. (2024). Establishing immortalized brown and white preadipocyte cell lines from young and aged mice. Current Protocols, 4, e70072. doi:10.1002/cpz1.70072
