| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IMPA1 recombinant protein (Position: M1-D277) was used as the immunogen for the IMPA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IMPA1 Antibody / Inositol monophosphatase 1 is a anti-IMPA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: IMPA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
IMPA1 is expressed abundantly in brain, kidney, and liver, reflecting its central role in inositol metabolism. In neurons, IMPA1 supports the phosphoinositide cycle, enabling sustained neurotransmitter signaling via inositol trisphosphate and diacylglycerol pathways. Pharmacologically, IMPA1 is a well-known molecular target of lithium, a mood-stabilizing drug used in the treatment of bipolar disorder. Lithium inhibits IMPA1 activity, leading to inositol depletion and modulation of neuronal signaling, supporting the �inositol depletion hypothesis� for mood stabilization.
The human IMPA1 gene is located on chromosome 8q21.13 and encodes a 277-amino acid protein that functions as a homodimer. The enzyme binds magnesium ions within its active site to hydrolyze phosphate from a range of inositol monophosphate substrates. Structural studies have revealed conserved residues critical for catalysis and lithium binding. Genetic or functional alterations in IMPA1 have been associated with neurological disorders, cognitive deficits, and certain metabolic diseases. Experimental evidence also implicates IMPA1 in maintaining cellular osmotic balance, particularly in renal epithelial cells where inositol acts as a compatible osmolyte.
Immunohistochemical analysis using IMPA1 antibody shows strong cytoplasmic staining in neurons, glial cells, renal tubules, and hepatocytes. The antibody is useful for studying inositol phosphate metabolism, lithium pharmacology, and neuronal signaling pathways. IMPA1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
