| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ING3 recombinant protein (Position: M1-Q367) was used as the immunogen for the ING3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ING3 Antibody / Inhibitor of growth protein 3 is a anti-ING3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ING3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Inhibitor of growth protein 3 contains a conserved plant homeodomain (PHD) finger that recognizes trimethylated histone H3 at lysine 4 (H3K4me3), a hallmark of transcriptionally active chromatin. By binding to this epigenetic mark, ING3 recruits acetyltransferase complexes such as TIP60 (KAT5), promoting histone acetylation and transcriptional activation of target genes involved in cell cycle arrest, DNA repair, and apoptosis. The ING3 antibody allows direct visualization of protein localization and quantification of expression levels across tissues and cell types, supporting studies of ING3-dependent transcriptional control.
As a tumor suppressor, Inhibitor of growth protein 3 plays a crucial role in preventing malignant transformation. Downregulation or mutation of ING3 has been reported in multiple cancers, including prostate, head and neck, and hepatocellular carcinoma. Loss of expression correlates with reduced apoptosis and enhanced proliferation. Researchers use the ING3 antibody to assess protein expression in cancer specimens and cell models, providing insights into its role in tumorigenesis and epigenetic misregulation. Restoration of ING3 activity has been shown to sensitize cells to apoptosis and DNA-damaging agents, emphasizing its therapeutic relevance.
Beyond tumor suppression, Inhibitor of growth protein 3 contributes to p53-dependent transcription and DNA repair processes. It interacts with the p53 tumor suppressor to enhance transcriptional activation of pro-apoptotic genes such as BAX and PUMA. The ING3 antibody supports studies aimed at mapping these protein–protein interactions and elucidating pathways linking chromatin remodeling to cellular stress responses. In addition, ING3 has been implicated in developmental regulation and differentiation, suggesting functions beyond its canonical tumor suppressor role.
The ING3 antibody is suitable for use in western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation. These assays allow researchers to examine ING3 expression dynamics in response to cellular stimuli or drug treatment.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
