| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human INPP5F recombinant protein (Position: H317-Q1112) was used as the immunogen for the INPP5F antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
INPP5F Antibody / Type II inositol 1,4,5-trisphosphate 5-phosphatase is a anti-INPP5F Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: INPP5F
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
INPP5F localizes to the cytoplasm and Golgi apparatus and is expressed in multiple tissues, with highest levels in heart, brain, and skeletal muscle. Functional studies have demonstrated that INPP5F attenuates insulin and growth factor signaling by dephosphorylating PIP3, thereby reducing AKT activation. In cardiomyocytes, INPP5F serves as an intrinsic brake on hypertrophic signaling by modulating the PI3K/AKT/mTOR axis. Mice lacking INPP5F display enhanced cardiac growth and increased susceptibility to heart failure, underscoring its physiological importance in maintaining normal cardiac morphology and function.
The INPP5F antibody is widely used in signal transduction, cardiovascular, and metabolic research to study phosphoinositide regulation and AKT signaling. Western blot analysis typically reveals a 108 kilodalton band corresponding to the full-length enzyme. Immunofluorescence shows perinuclear and Golgi-associated staining patterns consistent with its subcellular localization. Because INPP5F interacts with lipid-binding proteins and signaling kinases, this antibody is also employed in co-immunoprecipitation and pathway-mapping assays. Reduced INPP5F expression has been observed in glioblastoma, prostate cancer, and other tumors, suggesting its function as a tumor suppressor through attenuation of PI3K-driven oncogenic pathways.
In addition to its phosphatase activity, INPP5F contributes to endosomal sorting and receptor recycling by shaping local phosphoinositide pools on endomembranes. It cooperates with SHIP2 and synaptojanin to balance phosphoinositide turnover in response to receptor activation. The INPP5F antibody enables detailed examination of these signaling processes and provides insight into how lipid signaling defects contribute to disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
