| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | An E.coli-derived mouse recombinant protein (amino acids L45-Q347) was used as the immunogen for the Interferon gamma receptor 1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Interferon gamma receptor 1 Antibody / Ifngr1 is an antibody targeting Interferon gamma receptor 1, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: Interferon gamma receptor 1.
- Antibody identity: Polyclonal (rabbit origin); Rabbit IgG.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Mouse, Rat.
- Listed applications: WB, ELISA (refer to on-page specifications for application-specific guidance).
Biological background
Interferon gamma receptor 1 (IFNGR1) also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. IFN-gamma plays key roles in both the innate and adaptive immune response. IFN-gamma activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells. IFN-gamma production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-gamma production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation. Due to differences in the degree of glycosylation, there are 3 forms of IFN-gamma, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE. The anti-viral activity of IFN-gamma is due to its induction of PKR and other regulatory proteins. Binding of IFN-gamma to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-gamma dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1. The critical role of IFN-gamma in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-gamma or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-gamma also appears to have a role in atherosclerosis.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
