| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IQCE recombinant protein (Position: D112-H621) was used as the immunogen for the IQCE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IQCE Antibody / IQ domain-containing protein E is a anti-IQCE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: IQCE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
IQCE is encoded by the IQCE gene located on human chromosome 7p22.3. The protein contains one or more IQ motifs, short consensus sequences that mediate calcium-calmodulin interactions. These motifs enable IQCE to sense calcium changes and respond by altering its binding partners and structural conformation. It interacts with EF-hand calcium-binding protein EFCAB1 to form a complex required for the stability of the ciliary base. This partnership regulates Hedgehog signaling, a pathway central to embryogenesis and tissue differentiation. Disruption of IQCE causes ciliopathies, including acrocallosal syndrome, characterized by brain malformation, craniofacial defects, and polydactyly.
With the IQCE antibody, western blot analysis typically reveals a 77 kilodalton band, while immunofluorescence shows punctate staining at centrosomes and basal bodies. Ciliary localization is confirmed through co-staining with gamma-tubulin and acetylated tubulin. Loss-of-function studies demonstrate that IQCE depletion shortens or eliminates primary cilia, disrupting Hedgehog target gene activation. The protein also supports the recruitment of intraflagellar transport (IFT) components, ensuring proper axonemal elongation.
Beyond ciliary biology, IQCE expression in epithelial and fibroblast cells suggests additional roles in vesicular trafficking, cytoskeletal organization, and mechanosensory signaling. Its calcium sensitivity allows IQCE to integrate mechanical and biochemical cues, influencing organ development and tissue maintenance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
