| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IQCM recombinant protein (Position: R161-E501) was used as the immunogen for the IQCM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IQCM Antibody / IQ motif-containing protein M is a anti-IQCM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: IQCM
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Structurally, IQCM is characterized by its multiple IQ calmodulin-binding motifs, which enable dynamic regulation of protein conformation in response to intracellular calcium levels. It localizes primarily to developing germ cells, particularly in the flagellar basal body and axoneme, suggesting a function in microtubule organization and motor protein regulation. IQCM may interact with dynein and kinesin complexes to ensure coordinated flagellar movement.
The IQCM antibody is widely used in reproductive biology, developmental genetics, and ciliary research to examine spermatogenesis, motility regulation, and structural assembly of axonemal complexes. Western blot analysis identifies a 115 kilodalton band corresponding to IQCM, while immunofluorescence shows strong staining in the sperm flagellum and cytoplasmic vesicles of differentiating spermatids. This antibody supports studies exploring the molecular machinery underlying cilia and flagella function.
Mutations or loss of IQCM lead to defective sperm motility and infertility in animal models, emphasizing its essential role in sperm tail formation. The calcium-calmodulin-dependent activity of IQCM links cellular signaling to mechanical movement, integrating metabolic and structural regulation in germ cell development. The IQCM antibody provides an effective reagent for dissecting these interactions at the molecular level.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
