| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human IQGAP2 was used as the immunogen for the IQGAP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IQGAP2 Antibody / IQ motif containing GTPase activating protein 2 is a anti-IQGAP2 Rabbit antibody Recombinant Rabbit Monoclonal clone 30I08 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: IQGAP2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30I08, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
IQGAP2 antibody is widely used in cancer biology, hepatology, and cytoskeletal research. IQGAP2 is highly expressed in liver, stomach, and testis. It regulates actin dynamics and adherens junctions by interacting with beta catenin, E cadherin, and F actin. Altered expression of IQGAP2 has been linked to hepatocellular carcinoma and gastric cancer, where loss of IQGAP2 promotes tumorigenesis. By detecting IQGAP2, researchers can study its tumor suppressor functions and its role in epithelial tissue integrity.
Western blot assays with IQGAP2 antibody detect protein bands in hepatic and epithelial tissues. Immunohistochemistry highlights expression in liver lobules and gastric mucosa, while immunofluorescence reveals cytoplasmic localization at cell junctions and actin filaments. ELISA enables quantification of IQGAP2 expression across experimental samples.
IQGAP2 participates in multiple signaling pathways, including Wnt, MAPK, and PI3K AKT. Its ability to bind both actin and signaling proteins positions it as a key scaffold at the plasma membrane. In cancer, IQGAP2 expression is frequently downregulated, contrasting with IQGAP1, which is often upregulated. This differential regulation underscores the unique tumor suppressive properties of IQGAP2. By applying IQGAP2 antibody, scientists can investigate how cytoskeletal regulation intersects with cancer biology and metabolic homeostasis.
IQGAP2 also plays roles in immune signaling and reproduction. In immune cells, it modulates cytoskeletal dynamics during migration and activation. In testis, IQGAP2 expression suggests involvement in spermatogenesis and germ cell polarity. These diverse roles make IQGAP2 antibody a valuable reagent for broad research areas beyond oncology.
IQGAP2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
