| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IRAK4 recombinant protein (Position: N15-S460) was used as the immunogen for the IRAK4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IRAK4 Antibody / Interleukin-1 receptor-associated kinase 4 is a anti-IRAK4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: IRAK4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, IRAK4 contains an N-terminal death domain that mediates interaction with MyD88 and IRAK1, a kinase domain responsible for autophosphorylation, and regulatory regions that ensure signal specificity. IRAK4 acts as the first kinase activated in the TLR/IL-1R pathway, enabling the assembly of the Myddosome complex. This signaling cascade is essential for early innate immune responses against bacterial and viral pathogens.
The IRAK4 antibody is widely used in immunology, inflammation, and infection research to study signal transduction, cytokine regulation, and kinase activation. Western blot analysis detects a 52 kilodalton band corresponding to IRAK4, while immunofluorescence and immunohistochemistry show cytoplasmic staining in immune and epithelial cells. This antibody supports characterization of TLR signaling and innate immune responses in both normal and pathological states.
Deficiency or loss-of-function mutations in IRAK4 cause primary immunodeficiency characterized by impaired IL-1 and TLR signaling and recurrent bacterial infections. Conversely, overactivation contributes to chronic inflammation, autoimmune diseases, and cancer. The IRAK4 antibody provides a reliable tool for exploring innate immune signaling and therapeutic kinase targeting.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
