| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IRGQ recombinant protein (Position: D7-Q623) was used as the immunogen for the IRGQ antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IRGQ Antibody / Immunity-related GTPase family Q protein is a anti-IRGQ Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: IRGQ
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, IRGQ antibody identifies a 409-amino-acid GTP-binding protein localized mainly to the endoplasmic reticulum and Golgi apparatus. IRGQ hydrolyzes GTP to drive conformational changes that remodel intracellular membranes during immune activation and stress response. It is induced by interferon signaling and contributes to vesicle trafficking between endoplasmic reticulum and Golgi compartments.
The IRGQ gene is located on chromosome 19q13.33 and is expressed in immune-related tissues such as spleen, liver, and macrophages. Through its role in intracellular trafficking and vesicle fusion, IRGQ coordinates membrane remodeling events necessary for protein secretion and immune signaling.
Pathologically, IRGQ dysregulation has been associated with inflammatory and infectious diseases. Reduced IRGQ activity may impair vesicle transport, while overactivation can affect Golgi organization and cytokine secretion. Research using IRGQ antibody supports studies in GTPase biology, membrane dynamics, and immune regulation.
IRGQ antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect small GTPases and membrane-associated enzymes.
Structurally, Immunity-related GTPase family Q protein features conserved GTP-binding motifs (P-loop, switch I and II regions) and a C-terminal amphipathic helix that mediates membrane association. This antibody facilitates detailed study of IRGQ's GTPase-dependent mechanisms in host defense and vesicular transport.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
