| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
The interferon-stimulated response element (ISRE) is a conserved DNA motif that mediates transcriptional responses to type I interferons such as IFN-alpha and IFN-beta. Engagement of the interferon receptor activates the receptor-associated Janus kinases JAK1 and TYK2, which phosphorylate STAT proteins. Phosphorylated STAT1 and STAT2 combine with IRF9 to form the ISGF3 complex, which binds the ISRE and drives expression of hundreds of interferon-stimulated genes. This JAK/STAT axis governs antiviral defense, innate immune signaling, and inflammatory responses, and its dysregulation is linked to viral infection, autoimmune disease, and cancer immune evasion, making ISRE activity a central readout in immunology research.
Product Description & Applications
The ISRE Reporter Lentivirus places a reporter gene under the control of tandem interferon-stimulated response elements coupled to a minimal promoter, providing a sensitive readout of JAK/STAT pathway activation and IFN-alpha-induced gene expression. Reporter options include fluorescent (GFP, RFP), luminescent (firefly and Renilla luciferase), and secreted (SEAP) formats, with puromycin or blasticidin selection for rapid generation of stable reporter cell lines. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring interferon signaling, screening modulators of the JAK/STAT pathway, and studying antiviral and inflammatory responses.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the JAK/STAT pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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