| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant mouse protein (amino acids F20-E1061) was used as the immunogen for the Itgae antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Itgae Antibody / Cd103 is an antibody targeting ITGAE, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: ITGAE (reported localization: Cell membrane, cytoplasm).
- Antibody identity: Polyclonal (rabbit origin); Rabbit IgG.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Mouse, Rat.
- Listed applications: IHC-P, Direct ELISA (refer to on-page specifications for application-specific guidance).
Biological background
ITGAE (INTEGRIN, ALPHA-E), also known as CD103, is an integrin protein that in human is encoded by the ITGAE gene. The ITGAE gene is mapped to chromosome 17p13 by inclusion within a BAC contig. ITGAE is expressed widely on intraepithelial lymphocyte (IEL) T cells (both alpha-beta T cells and gamma-delta T cells) and on some peripheral regulatory T cells(Tregs) which is important for decreasing the immune response and appears to play a crucial role in the prevention of autoimmune diseases. ITGAE shares overall homology with other alpha integrins but has a unique 55-amino acid extra region located just N-terminal to the I domain that contains 18 consecutive charged residues and a proteolytic cleavage site.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
