| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ITM2C recombinant protein (Position: E33-D226) was used as the immunogen for the ITM2C antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ITM2C Antibody / Integral membrane protein 2C is a anti-ITM2C Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ITM2C
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, ITM2C contains a short cytoplasmic N-terminal tail, a single transmembrane domain, and a large luminal BRICHOS domain that assists in proper folding of amyloidogenic proteins. ITM2C interacts with APP and modulates its processing by influencing the activity of secretases, thereby reducing amyloid beta peptide production. This regulatory function positions ITM2C as a potential protective factor against neurodegenerative diseases such as Alzheimer's disease.
The ITM2C antibody is widely used in neuroscience and neurodegeneration research to study synaptic organization, APP metabolism, and BRICHOS domain biology. Western blot analysis detects a 30 kilodalton band corresponding to ITM2C, while immunofluorescence reveals Golgi and vesicular localization within neurons and glial cells. This antibody supports investigations into the cellular pathways controlling amyloid processing and synaptic stability.
Loss or dysregulation of ITM2C has been associated with cognitive decline and neuroinflammation. Reduced expression leads to impaired APP trafficking and enhanced amyloid accumulation, while overexpression protects against oxidative and excitotoxic stress. Beyond the brain, ITM2C is expressed in pancreatic islets, suggesting additional roles in endocrine regulation. The ITM2C antibody provides a powerful tool for elucidating mechanisms of protein homeostasis and synaptic maintenance in health and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
