| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IVNS1ABP recombinant protein (Position: Q30-D580) was used as the immunogen for the IVNS1ABP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IVNS1ABP Antibody / Influenza virus NS1A-binding protein is a anti-IVNS1ABP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: IVNS1ABP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
IVNS1ABP is encoded by the IVNS1ABP gene on human chromosome 1p13.3 and produces a protein of approximately 72 kDa. The protein contains multiple WD40 repeats that mediate protein-protein interactions and a nuclear localization signal that directs it to nucleoplasmic and speckle regions. IVNS1ABP interacts with heterogeneous nuclear ribonucleoproteins (hnRNPs), transcriptional regulators, and chromatin remodelers such as BRG1. Its diverse set of interactions positions it as a hub protein coordinating mRNA maturation and transport.
Research using the IVNS1ABP antibody shows that it contributes to the regulation of pre-mRNA splicing, ribosome biogenesis, and stress granule dynamics. Upon viral infection, IVNS1ABP binds to influenza NS1A protein, enhancing viral mRNA processing and nuclear export. However, under normal conditions, it supports cell survival and proliferation through stabilization of specific RNA-protein complexes. Western blot analysis typically identifies a band near 90 kDa, while immunofluorescence reveals nuclear and nucleolar staining. Loss of IVNS1ABP disrupts splicing patterns and increases sensitivity to stress-induced apoptosis.
Beyond viral interactions, IVNS1ABP is linked to cell differentiation and cytoskeletal organization. It modulates actin dynamics by interacting with profilin and other actin-binding proteins, connecting nuclear signaling with cytoplasmic architecture. Dysregulation of IVNS1ABP has been implicated in developmental disorders and certain cancers.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
