| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PHF17/JADE1 recombinant protein (Position: I93-D811) was used as the immunogen for the JADE1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
JADE1 Antibody / Jade family PHD finger protein 1 / PHF17 is a anti-JADE1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: JADE1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, JADE1 contains two PHD (plant homeodomain) zinc finger motifs, an N-terminal coiled-coil domain, and a C-terminal PEST sequence important for protein stability. These domains facilitate chromatin binding and complex formation with HBO1, ING4, and BRPF family proteins. JADE1 belongs to the chromatin-modifying family of histone acetylation regulators that modulate epigenetic transcriptional control. Co-localization studies show JADE1 primarily localized to the nucleus, especially at transcriptionally active chromatin regions.
Functionally, JADE1 promotes histone acetylation and chromatin accessibility, facilitating the expression of genes involved in cell cycle regulation, epithelial morphogenesis, and DNA repair. It stabilizes the HBO1 complex and coordinates acetyltransferase activity at replication origins and promoters. In kidney epithelial cells, JADE1 regulates Wnt and TGF-beta pathways to maintain differentiation and suppress epithelial-to-mesenchymal transition (EMT). JADE1 also interacts with von Hippel-Lindau (VHL) tumor suppressor protein, enhancing the degradation of oncoproteins such as beta-catenin and cyclin E, linking it to tumor suppression mechanisms.
Loss or mutation of JADE1 disrupts chromatin organization and promotes tumorigenesis, particularly in renal cell carcinoma and hepatocellular carcinoma. Reduced JADE1 levels correlate with increased proliferation and dedifferentiation of epithelial cells. Pathway associations include histone acetylation, transcriptional regulation, and Wnt signaling. During development, JADE1 expression supports epithelial lineage specification and organogenesis. Known binding partners include HBO1, ING4, BRPF3, and VHL, forming multi-protein chromatin remodeling assemblies.
The JADE1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
