| Field | Specification |
|---|---|
| Target | |
| Alternative names | MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mi |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. AA range:151-200 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes JNK1/2/3 (phospho Tyr185). It is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA.
Validated Applications
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
|---|---|
| IF | 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. AA range:151-200
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185. AA range:151-200 |
| Concentration | 1 mg/mL |
| Observed Band | 45kD |
| Subcellular Location | Cytoplasm . Nucleus . Cell junction, synapse . In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus. . |
| Swiss-Prot | P45983, P45984, P53779 |
| Gene ID | 5599, 5601, 5602 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for JNK1/2/3 (phospho Tyr185) research from ELK Biotechnology, or contact us for alternative formats.
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