KBTBD2 Antibody

Overview

KBTBD2 Antibody is a research-use antibody directed against KBTBD2. It is supplied for use in common immunoassay contexts such as WB, IF/ICC, FACS (RUO).

Key elements and design rationale

• Target: KBTBD2.
• Description (provided): encodes a BTB-Kelch family subunit that p85 alpha-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85 alpha protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling.
• Antibody type: Rabbit, Polyclonal (rabbit origin), Rabbit IgG.
• Format: Antigen affinity purified; Antigen affinity purified.
• Species reactivity: tested: Human, Mouse, Rat.
• Immunogen (if provided): Amino acids QYDLELDRWSLRQHISERVLWDLGRDFR from the human protein were used as the immunogen for the KBTBD2 antibody..

The information above helps you match the antibody format to your assay context, interpret species-dependent differences, and anticipate how epitope context (isoforms, PTMs, or conformational state) may influence signal.

Biological background

Kelch repeat and BTB domain containing 2 (KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85 alpha-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85 alpha protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling. However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization. In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity and insulin sensitivity.

For curated annotations (gene/protein naming, domains, isoforms, and pathway links) for KBTBD2, consult primary databases such as UniProt, NCBI Gene, and Ensembl.

Research relevance and current trends

• Context-dependent expression studies: researchers often examine KBTBD2 abundance and localization across perturbations (genetic, pharmacologic, or environmental) to connect phenotype to molecular changes.
• Reagent reproducibility: there is growing emphasis on antibody specificity checks using orthogonal approaches (e.g., genetic perturbation or independent antibodies) and transparent reporting of clone/lot information.
• Multi-modal datasets: antibody-based readouts are increasingly combined with transcriptomics and imaging to relate protein-level measurements to cell-state transitions.

Common research applications

• Western blotting (immunoblot) for relative detection of target protein abundance and apparent molecular weight.
• Immunofluorescence for subcellular localization and cell-type specific expression patterns.
• FACS: commonly used to detect or compare KBTBD2 across experimental conditions (conceptual guidance only).

When comparing conditions, interpret changes in signal in the context of sample composition, expected localization, and any known isoform complexity for the target.

Notes for experimental interpretation

• Isoforms and PTMs: alternative splicing or post-translational modifications can change epitope accessibility and apparent molecular weight; interpret bands/signals accordingly.
• Cross-reactivity and matrix effects: background binding can vary by sample type, species, and blocking/detection chemistries; include appropriate negative controls.
• Control concepts: where feasible, use genetic perturbation (KO/KD/overexpression), orthogonal assays, or independent antibodies to support specificity claims.

Antibody considerations: Polyclonal reagents may recognize multiple epitopes and can increase sensitivity but may show broader binding profiles, while monoclonal clones provide a single-epitope readout that can improve consistency across experiments. If a conjugate is listed, the antibody supports more direct detection workflows; otherwise, it is typically used with a compatible secondary antibody.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.