| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human KCTD9 was used as the immunogen for the KCTD9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
KCTD9 Antibody / Potassium channel tetramerization domain containing protein 9 is a anti-KCTD9 Rabbit antibody Recombinant Rabbit Monoclonal clone 29K82 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: KCTD9
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29K82, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
Although originally identified through sequence similarity to potassium channel subunits, KCTD9 does not form ion channels. Research using KCTD9 antibody has demonstrated that it functions as an adaptor protein in ubiquitin ligase complexes, targeting substrates for ubiquitination and degradation. By regulating protein turnover, KCTD9 influences signaling cascades and cellular homeostasis.
Studies with KCTD9 antibody have revealed important roles in immune function. KCTD9 has been implicated in natural killer cell activation, where it regulates cytokine production and cytotoxic responses. Dysregulation of KCTD9 expression impairs antiviral immunity and contributes to immune mediated pathology. This highlights its relevance in both protective and pathogenic immune responses.
KCTD9 has also been linked to apoptosis. Research using KCTD9 antibody has shown that altered expression modulates mitochondrial pathways of cell death, influencing susceptibility to stress and chemotherapeutic agents. These findings suggest that KCTD9 participates in cross talk between ubiquitin signaling and apoptosis pathways.
Dysregulation of Potassium channel tetramerization domain containing protein 9 has been associated with disease. Elevated expression is observed in inflammatory conditions and cancers, while reduced expression may compromise immune defense. These context dependent roles underscore the importance of precise regulation of KCTD9 levels.
KCTD9 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects endogenous levels across tissues, immunohistochemistry reveals localization in immune organs, and immunofluorescence demonstrates cytoplasmic distribution. These experimental applications make KCTD9 antibody a versatile reagent in signaling and immunology research.
By supplying validated KCTD9 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
