| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human KDM8 recombinant protein (Position: D4-D406) was used as the immunogen for the KDM8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
KDM8 Antibody / Lysine-specific demethylase 8 is a anti-KDM8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: KDM8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
KDM8, also known as JMJD5, is a member of the Jumonji C (JmjC) domain-containing family of Fe(II)- and 2-oxoglutarate-dependent oxygenases. It localizes to the nucleus and functions both as a histone demethylase and as a cofactor for cell cycle regulatory proteins such as CDK1. Through these activities, KDM8 coordinates chromatin dynamics and ensures proper metaphase alignment during mitosis.
The KDM8 antibody is widely used in epigenetics, developmental biology, and cancer research to study histone methylation control, transcriptional regulation, and chromatin remodeling. Western blot analysis detects a 47 kilodalton band corresponding to KDM8, while immunofluorescence reveals nuclear staining consistent with its chromatin-associated functions. This antibody enables examination of histone modification patterns and their effects on gene expression and cell division.
Aberrant expression of KDM8 has been linked to oncogenesis, particularly in breast and prostate cancers, where it promotes proliferation and cell cycle progression by modulating chromatin accessibility. KDM8 also interacts with p53 and ribosomal proteins to coordinate metabolic and stress responses. The KDM8 antibody provides a powerful tool for studying epigenetic regulation and transcriptional dynamics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
